Tang Boping, Zhou Kaiya, Song Daxiang, Yang Guang, Dai Aiyun
Institute of Genetic Resources, College of Life Sciences, Nanjing Normal University, Nanjing 210097, China.
Mol Phylogenet Evol. 2003 Nov;29(2):309-16. doi: 10.1016/s1055-7903(03)00112-x.
To help resolve phylogenetic relationships among the mitten crabs, complete sequences of the nuclear DNA internal transcribed spacer (ITS) and portions of the mitochondrial genome corresponding to the cytochrome oxidase I (COI), were sequenced for all Asian mitten crabs of the genus Eriocheir and seven species of the Grapsoidea. The resulting phylogeny supports the establishment of a separate genus Neoeriocheir, but does not provide justification for the recognition of Platyeriocheir. A female mitten crab specimen from the Zhujiang River, China, was considered to be Eriocheir recta (), a species previously synonymized with Eriocheir japonica (de Haan, 1835). In the ITS analysis, a sequence from Eriocheir formosa (from Taiwan) falls within a well-supported E. recta group, which indicates that E. formosa may have to be synonymized with E. recta. Three previously recognized members of the genus, E. japonica, Eriocheir sinensis, and Eriocheir hepuensis constitute a monophyletic sister group to E. recta in all phylogenetic trees. We provide evidence for the conspecific status of these taxa. Phylogenetic trees based on COI and combined COI and ITS sequences indicate that E. japonica consists of three subgroups. Since the name E. japonica (de Haan, 1835) takes precedence over E. sinensis (H. Milne Edwards, 1853) and E. hepuensis, we suggest that these three subgroups correspond to three subspecies of E. japonica: E. j. japonica, E. j. sinensis, and E. j. hepuensis.
为了帮助厘清绒螯蟹之间的系统发育关系，对中华绒螯蟹属的所有亚洲绒螯蟹以及方蟹科的7个物种的核DNA内转录间隔区（ITS）完整序列和与细胞色素氧化酶I（COI）对应的线粒体基因组部分进行了测序。所得系统发育树支持建立一个单独的新绒螯蟹属，但没有为认可平绒螯蟹属提供依据。来自中国珠江的一只雌性绒螯蟹标本被认为是直额绒螯蟹（Eriocheir recta），该物种先前被视为日本绒螯蟹（de Haan，1835）的同物异名。在ITS分析中，来自台湾的台湾绒螯蟹（Eriocheir formosa）的一个序列落在一个得到充分支持的直额绒螯蟹类群内，这表明台湾绒螯蟹可能不得不被视为直额绒螯蟹的同物异名。该属先前认可的三个成员，日本绒螯蟹、中华绒螯蟹和合浦绒螯蟹，在所有系统发育树中都构成直额绒螯蟹的一个单系姐妹类群。我们提供了这些分类单元同种地位的证据。基于COI以及COI和ITS联合序列的系统发育树表明，日本绒螯蟹由三个亚群组成。由于日本绒螯蟹（de Haan，1835）的名称优先于中华绒螯蟹（H. Milne Edwards，1853）和合浦绒螯蟹，我们建议这三个亚群对应于日本绒螯蟹的三个亚种：日本绒螯蟹指名亚种、日本绒螯蟹中华亚种和日本绒螯蟹合浦亚种。
