Lucas Annick, Kremer Eric J, Hemmi Silvio, Luis José, Vignon Françoise, Lazennec Gwendal
INSERM U540 Molecular and Cellular Endocrinology of Cancers, 60, rue de Navacelles, Montpellier 34090, France.
Biochem Biophys Res Commun. 2003 Oct 3;309(4):1011-6. doi: 10.1016/j.bbrc.2003.08.101.
Defining the ideal vectors to transduce breast cancer using viruses is currently under intense pre-clinical evaluation. Our study constitutes the first direct comparison of the infection efficiencies of a human serotype 5 (Ad5), a canine serotype 2 (CAV-2) adenovirus, and a human serotype 2 adeno-associated virus (AAV-2) in breast cancer cells. We observed an excellent infection efficiency for Ad5 vector, whereas both CAV-2 and AAV-2 vectors lead to low infection of these cells. Real-time PCR, flow cytometry, and antibody blocking studies suggest that Ad5 and CAV-2 infection ability is not strictly dependent on coxsackie adenovirus receptor (CAR) or alpha(v) integrin levels. In conclusion, our data suggest that human adenoviruses are excellent transducers of breast cancer cells, though it may be difficult to predict the extent of infection solely on CAR or alpha(v) integrin levels.
