Xia Yun, Gross Guenter W
Department of Biological Sciences and Center for Network Neuroscience, P.O. Box 305220, University of North Texas, Denton, TX 76203, USA.
Alcohol. 2003 Jul;30(3):167-74. doi: 10.1016/s0741-8329(03)00135-6.
Embryonic murine neuronal networks cultured on substrate-integrated microelectrode arrays were used to quantify acute electrophysiological effects of ethanol by using extracellular, multichannel recording of action potentials. Spontaneously active frontal cortex cultures showed repeatable, concentration-dependent sensitivities to ethanol, with initial inhibition at 20 mM and a spike rate 50% effective concentration (EC50) of 48.8+/-5.4 mM. Ethanol concentrations of greater than 100 mM led to cessation of activity. The ethanol inhibitions up to the maximum tested 160 mM were reversible. Although ethanol did not change the shape of action potentials, unit-specific spike pattern effects were found. At 40 mM, ethanol decreased neuronal firing in 71%, increased firing in 20%, and generated no effect in 9% of all units observed (14 cultures, 200 discriminated units). The effects of combined application of ethanol and fluoxetine were additive. Excellent agreement with findings obtained from experimental studies with animals validates the use of these in vitro systems for alcohol research.
在基底集成微电极阵列上培养的胚胎小鼠神经元网络，通过细胞外多通道记录动作电位来量化乙醇的急性电生理效应。自发活跃的额叶皮质培养物对乙醇表现出可重复的、浓度依赖性敏感性，在20 mM时开始受到抑制，峰值频率50%有效浓度（EC50）为48.8±5.4 mM。乙醇浓度大于100 mM会导致活动停止。高达测试最大值160 mM的乙醇抑制作用是可逆的。虽然乙醇没有改变动作电位的形状，但发现了单位特异性的峰值模式效应。在40 mM时，乙醇使71%的神经元放电减少，20%的神经元放电增加，在所有观察到的单位（14个培养物，200个分辨单位）中，9%没有受到影响。乙醇和氟西汀联合应用的效果是相加的。与动物实验研究结果的高度一致性验证了这些体外系统在酒精研究中的应用。
