Ospina S S, Lopez-Munguia A, Gonzalez R L, Quintero R
Departamento de Farmacia, Instituto de Biotecnología, Universidad Nacional de Colombia, Bogotá.
J Chem Technol Biotechnol. 1992;53(2):205-14. doi: 10.1002/jctb.280530217.
A complete characterization of a penicillin acylase biocatalyst is presented, including the determination of physicochemical and kinetic parameters. Stability studies are detailed in terms of both storage temperature and pH as well as operational stability after 150 batch reactions of two hours duration each. An Arrhenius-type model was used to simulate the effect of pH on biocatalyst stability. A kinetic model is proposed to describe batch and continuous stirred tank reactors and to predict the long-term behavior of the process.
本文介绍了青霉素酰化酶生物催化剂的完整表征，包括物理化学和动力学参数的测定。从储存温度和pH值以及在150次每次持续两小时的间歇反应后的操作稳定性方面详细阐述了稳定性研究。使用了阿累尼乌斯型模型来模拟pH值对生物催化剂稳定性的影响。提出了一个动力学模型来描述间歇式和连续搅拌釜式反应器，并预测该过程的长期行为。
