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引力的变化会诱导拟南芥幼苗中基因表达的改变。

Changes in gravitational forces induce modifications of gene expression in A. thaliana seedlings.

作者信息

Centis-Aubay S, Gasset G, Mazars C, Ranjeva R, Graziana A

机构信息

Signaux et Messages Cellulaires chez les Végétaux, Centre National de la Recherche Scientifique, Université Paul Sabatier, 24 chemin de Borde Rouge, Auzeville, 31326 Castanet-Tolosan, France.

出版信息

Planta. 2003 Dec;218(2):179-85. doi: 10.1007/s00425-003-1103-7. Epub 2003 Sep 12.

DOI:10.1007/s00425-003-1103-7
PMID:13680230
Abstract

By comparing the expression patterns of selected genes from Arabidopsis thaliana (L.) Heynh. grown either at 1 g or on a clinostat (horizontally or vertically inverted, 1 rpm), and either used directly or after hypergravity stimulation, we have shown that the pattern of expression did not proceed in a stereotypical manner. Rather, the selected genes fell into different classes. These classes include (i) those insensitive to the gravitational conditions, (ii) those that are regulated in an opposite manner by hypergravity and clinostat conditions, (iii) those that are desensitised to hypergravity by long-term culture on a clinostat, and (iv) those enhanced by such a treatment. Our data suggest that rapid reorientation of gene expression is likely to occur in response to changes in the gravitational conditions.

摘要

通过比较拟南芥(Arabidopsis thaliana (L.) Heynh.)在1g条件下生长、在回转器上(水平或垂直倒置,1转/分钟)生长,以及直接使用或在超重力刺激后使用时所选基因的表达模式,我们发现基因表达模式并非以固定模式进行。相反,所选基因可分为不同类别。这些类别包括:(i)对重力条件不敏感的基因;(ii)在超重力和回转器条件下以相反方式调控的基因;(iii)通过在回转器上长期培养而对超重力脱敏的基因;以及(iv)经此类处理后表达增强的基因。我们的数据表明,基因表达可能会因重力条件的变化而迅速重新定向。

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1
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Planta. 2003 Dec;218(2):179-85. doi: 10.1007/s00425-003-1103-7. Epub 2003 Sep 12.
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本文引用的文献

1
The role of actin filaments in the gravitropic response of snapdragon flowering shoots.肌动蛋白丝在金鱼草开花枝条向重力性反应中的作用。
Planta. 2003 Apr;216(6):1034-42. doi: 10.1007/s00425-002-0957-4. Epub 2003 Jan 18.
2
Transcription profiling of the early gravitropic response in Arabidopsis using high-density oligonucleotide probe microarrays.利用高密度寡核苷酸探针微阵列对拟南芥早期向重力性反应进行转录谱分析。
Plant Physiol. 2002 Oct;130(2):720-8. doi: 10.1104/pp.009688.
3
Requirement of the Auxin Polar Transport System in Early Stages of Arabidopsis Floral Bud Formation.
拟南芥幼苗在短期到长期 3-D 倾斜旋转暴露过程中的基因表达变化。
Planta. 2011 Aug;234(2):255-70. doi: 10.1007/s00425-011-1395-y. Epub 2011 Mar 18.
4
Changes in the effective gravitational field strength affect the state of phosphorylation of stress-related proteins in callus cultures of Arabidopsis thaliana.有效重力场强度的变化会影响拟南芥愈伤组织培养物中应激相关蛋白的磷酸化状态。
J Exp Bot. 2009;60(3):779-89. doi: 10.1093/jxb/ern324. Epub 2009 Jan 6.
5
Subnucleolar location of fibrillarin and NopA64 in Lepidium sativum root meristematic cells is changed in altered gravity.在重力改变的情况下,独行菜根分生组织细胞中纤维蛋白原和NopA64的核仁亚定位发生变化。
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The DMI1 and DMI2 early symbiotic genes of medicago truncatula are required for a high-affinity nodulation factor-binding site associated to a particulate fraction of roots.蒺藜苜蓿的DMI1和DMI2早期共生基因是与根的微粒部分相关的高亲和力结瘤因子结合位点所必需的。
Plant Physiol. 2006 Jan;140(1):365-73. doi: 10.1104/pp.105.068981. Epub 2005 Dec 23.
生长素极性运输系统在拟南芥花芽形成早期的需求
Plant Cell. 1991 Jul;3(7):677-684. doi: 10.1105/tpc.3.7.677.
4
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J Plant Growth Regul. 2002 Jun;21(2):137-45. doi: 10.1007/s003440010052. Epub 2002 May 24.
5
Reduced gravitropism in inflorescence stems and hypocotyls, but not roots, of Arabidopsis mutants with large plastids.具有大型质体的拟南芥突变体的花序茎和下胚轴中重力向性降低,但根中没有。
Physiol Plant. 2002 Apr;114(4):627-636. doi: 10.1034/j.1399-3054.2002.1140417.x.
6
Plants, plant pathogens, and microgravity--a deadly trio.植物、植物病原体与微重力——致命三重奏。
Gravit Space Biol Bull. 2001 Jun;14(2):15-23.
7
Gravity-stimulated changes in auxin and invertase gene expression in maize pulvinal cells.重力刺激对玉米叶枕细胞中生长素和转化酶基因表达的影响。
Plant Physiol. 2002 Feb;128(2):591-602. doi: 10.1104/pp.010579.
8
Reduced expression of alpha-tubulin genes in Arabidopsis thaliana specifically affects root growth and morphology, root hair development and root gravitropism.拟南芥中α-微管蛋白基因表达的降低特别影响根的生长和形态、根毛发育以及根的向地性。
Plant J. 2001 Oct;28(2):145-57. doi: 10.1046/j.1365-313x.2001.01142.x.
9
The effect of a microgravity (space) environment on the expression of expansins from the peg and root tissues of Cucumis sativus.微重力(太空)环境对黄瓜茎蔓和根组织中扩展蛋白表达的影响。
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