Kim Byoung-Hyoun, Lee Suk Chin, Lee Hye Jin, Ok Jong Hoa
Analytical and Computational Science, Research Park, LG Chemical Ltd, 104-1, Moonji-dong, Yusong-gu, Daejon, 305-380, South Korea.
Biomed Chromatogr. 2003 Sep;17(6):396-403. doi: 10.1002/bmc.254.
A pre-column derivatization liquid chromatographic method has been developed for the analysis of aminoglycoside antibiotics using phenylisocyanate as a derivatization reagent. Derivatives including kanamycin, neomycin and gentamicin were formed by reaction of the analytes with phenylisocyanate in the presence of triethylamine. Phenylisocyanato groups were attached to corresponding amino groups of aminoglycoside and their molecular mass was confirmed by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). The experimental conditions for derivatization and separation of aminoglycoside derivatives were optimized and validated. A simple liquid chromatographic method for the determination of aminoglycoside antibiotics was demonstrated.
已开发出一种柱前衍生化液相色谱法，以异氰酸苯酯作为衍生化试剂来分析氨基糖苷类抗生素。在三乙胺存在下，分析物与异氰酸苯酯反应形成包括卡那霉素、新霉素和庆大霉素在内的衍生物。异氰酸苯酯基团连接到氨基糖苷类的相应氨基上，其分子量通过液相色谱 - 电喷雾电离 - 质谱（LC - ESI - MS）得以确认。对氨基糖苷类衍生物的衍生化和分离实验条件进行了优化和验证。展示了一种用于测定氨基糖苷类抗生素的简单液相色谱方法。
