通过九个构件的组装实现人表皮生长因子（h-EGF）的全合成。

Total solution synthesis of human epidermal growth factor (h-EGF) by the assembly of nine building blocks.

作者信息

Shin S Y, Kaburaki Y, Watanabe M, Munekata E

机构信息

Institute of Applied Biochemistry, University of Tsukuba, Japan.

出版信息

Biosci Biotechnol Biochem. 1992 Mar;56(3):404-8. doi: 10.1271/bbb.56.404.

DOI:10.1271/bbb.56.404

PMID:1368324

Abstract

Human epidermal growth factor (h-EGF) composed of 53 amino acids bearing three intramolecular disulfide bridges was synthesized by the maximum protecting solution method. The synthetic h-EGF coincided with recombinant h-EGF by reverse-phase HPLC, and the sites of three intramolecular disulfide bridges were ascertained by a thermolytic digestion. The synthetic h-EGF possessed m/z 6215.7 in its FAB-MS as expected, and exhibited compatible mitogenic activity.

摘要

采用最大保护溶液法合成了由53个氨基酸组成、带有三个分子内二硫键的人表皮生长因子（h-EGF）。合成的h-EGF通过反相高效液相色谱法与重组h-EGF一致，并且通过热解消化确定了三个分子内二硫键的位置。合成的h-EGF在其快原子轰击质谱（FAB-MS）中具有预期的m/z 6215.7，并且表现出相当的促有丝分裂活性。