从cDNA推导的大鼠单胺氧化酶A的一级结构及其在大鼠组织中的表达。
Primary structure of rat monoamine oxidase A deduced from cDNA and its expression in rat tissues.
作者信息
Kuwahara T, Takamoto S, Ito A
机构信息
Department of Biology, Daiichi College of Pharmaceutical Sciences, Fukuoka, Japan.
出版信息
Agric Biol Chem. 1990 Jan;54(1):253-7.
The cDNA for rat monoamine oxidase A (MAO-A) mRNA was isolated from a liver cDNA library in lambda gt11 as the cDNA species cross-hybridizing with rat monoamine oxidase B (MAO-B) cDNA. The primary structure of the protein, deduced from the nucleotide sequence, consisted of 526 amino acid residues and its molecular mass was calculated to be 59.6 kD. The amino acid sequence shows about 70% identity with that of rat MAO-B. Northern blot analysis showed that MAO-B mRNA was expressed in various rat tissues and the highest expression was observed in heart. The MAO-B mRNA was quite different from MAO-A mRNA in the tissue-specific expression and liver had the highest level of the mRNA.
从λgt11载体的大鼠肝脏cDNA文库中分离出与大鼠单胺氧化酶B（MAO - B）cDNA交叉杂交的cDNA物种，即大鼠单胺氧化酶A（MAO - A）mRNA的cDNA。根据核苷酸序列推导的该蛋白质一级结构由526个氨基酸残基组成，其分子量经计算为59.6 kD。氨基酸序列与大鼠MAO - B的氨基酸序列显示约70%的同一性。Northern印迹分析表明，MAO - B mRNA在大鼠的各种组织中均有表达，在心脏中观察到最高表达。MAO - B mRNA在组织特异性表达方面与MAO - A mRNA有很大不同，肝脏中该mRNA水平最高。
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