灰色链霉菌几丁质酶对部分N-乙酰化壳聚糖的作用模式

Action pattern of Streptomyces griseus chitinase on partially N-acetylated chitosan.

作者信息

Ohtakara A, Matsunaga H, Mitsutomi M

机构信息

Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, Japan.

出版信息

Agric Biol Chem. 1990 Dec;54(12):3191-9.

PMID:1368638

Abstract

Oligosaccharides produced during the course of the hydrolysis of 25% N-acetylated chitosan by Streptomyces griseus chitinase were fractionated by CM-Sephadex C-25 and Toyopearl HW-40F column chromatographies. Sugar compositions and sequences of main oligosaccharides were identified by N-acetylation, exo-splitting with beta-GlcNAcase and beta-GlcNase, and nitrous acid degradation. In addition to N-acetylated saccharides, GlcNAc, (GlcNAc)2, and (GlcNAc)3, hetero-chitooligosaccharides such as GlcN.GlcNAc, GlcN.GlcNAc.GlcNAc, GlcN.GlcN.GlcNAc, GlcN.GlcNAc.GlcNAc.GlcNAc, GlcNAc.GlcN.GlcNAc.GlcNAc, GlcN.GlcNAc.GlcN.GlcNAc, and GlcN.GlcN.GlcNAc.GlcNAc were identified. These results indicate that Streptomyces griseus chitinase specifically cleaves the N-acetyl-beta-D-glucosaminidic linkages in partially N-acetylated chitosan.

摘要

用灰色链霉菌几丁质酶水解25% N-乙酰化壳聚糖过程中产生的寡糖，通过CM-葡聚糖凝胶C-25和Toyopearl HW-40F柱色谱进行分级分离。通过N-乙酰化、用β-葡萄糖苷酶和β-葡萄糖胺酶进行外切裂解以及亚硝酸降解，鉴定了主要寡糖的糖组成和序列。除了N-乙酰化糖类、GlcNAc、(GlcNAc)2和(GlcNAc)3外，还鉴定了杂合壳寡糖，如GlcN.GlcNAc、GlcN.GlcNAc.GlcNAc、GlcN.GlcN.GlcNAc、GlcN.GlcNAc.GlcNAc.GlcNAc、GlcNAc.GlcN.GlcNAc.GlcNAc、GlcN.GlcNAc.GlcN.GlcNAc和GlcN.GlcN.GlcNAc.GlcNAc。这些结果表明，灰色链霉菌几丁质酶特异性切割部分N-乙酰化壳聚糖中的N-乙酰-β-D-葡萄糖胺糖苷键。