Ben Trivedi A, Kitabatake N, Doi E
Research Institute for Food Science, Kyoto University, Japan.
Agric Biol Chem. 1990 Nov;54(11):2961-6.
The toxicity of dimethyl sulfoxide (Me2SO) was examined in HeLa cells cultured at 37 degrees C for up to 72 hr. The growth of the cells was measured by a colorimetric method with the use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), which gave good correlation between the cell number and the color development from the reduction of MTT under suitable conditions. When the initial number of cells was 3 x 10(4)/ml, Me2SO at 1% or less had no apparent effect on proliferation for up to 48 hr of incubation, but in longer incubations, cell growth was repressed. When the initial number of cells was 3 x 10(5)/ml, the effect of Me2SO was similar.
在37℃培养长达72小时的HeLa细胞中检测了二甲基亚砜（Me2SO）的毒性。通过使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）的比色法测量细胞生长，在合适条件下，MTT还原产生的颜色变化与细胞数量之间具有良好的相关性。当初始细胞数为3×10⁴/ml时，1%及以下的Me2SO在长达48小时的孵育中对增殖没有明显影响，但在更长时间的孵育中，细胞生长受到抑制。当初始细胞数为3×10⁵/ml时，Me2SO的作用相似。
