Hatase O, Tsutsui K, Oda T
Acta Med Okayama. 1976 Jun;30(3):147-52.
Phosphate-binding protein(s) was found in the inner mitochondrial membrane of calf heart by Sephadex G-200 and G-25 gel filtration. The binding activity was inhibited by N-ethylmaleimide and competed by a large amount of cold phosphate. The amount of phosphate bound to the fraction was 29 nmoles per mg of protein. Affinity chromatography with phosphate-bound Sepharose 4B confirmed the presence of phosphate-binding protein(s) in the active fraction of mitochondrial membrane fractionated by gel filtration.
通过葡聚糖凝胶G - 200和G - 25凝胶过滤，在小牛心脏的线粒体内膜中发现了磷酸盐结合蛋白。该结合活性被N - 乙基马来酰亚胺抑制，并被大量冷磷酸盐竞争。与该组分结合的磷酸盐量为每毫克蛋白质29纳摩尔。用磷酸结合的琼脂糖4B进行亲和层析，证实了在通过凝胶过滤分级分离的线粒体膜活性组分中存在磷酸盐结合蛋白。
