Kühnemund O, Köhler W
Allerg Immunol (Leipz). 1976;22(2):171-8.
Streptococci of group A, type 1, are lysed by use of phage associated lysin, which is characterized as a N-Acetyl-D-glucosaminidase. This enzyme has no proteolytic activity. Type specific protein antigens are isolated and purified by ion exchange chromatography on CM-cellulose and DEAE-cellulose of the crude extract, concentrated by precipitation with ammonium sulfate. The immunization tests are carried out on monkeys and rabbits.
A组1型链球菌可通过使用噬菌体相关溶素进行裂解,该溶素的特征为N-乙酰-D-葡糖胺酶。这种酶没有蛋白水解活性。通过在CM-纤维素和DEAE-纤维素上对粗提取物进行离子交换色谱法分离和纯化型特异性蛋白质抗原,并用硫酸铵沉淀进行浓缩。在猴子和兔子身上进行免疫试验。