Ladisch M R, Kohlmann K L
Laboratory of Renewable Resources Engineering, A. A. Potter Engineering Center, Purdue University, West Lafayette, Indiana 47907.
Biotechnol Prog. 1992 Nov-Dec;8(6):469-78. doi: 10.1021/bp00018a001.
Insulin is a well-characterized peptide that can be produced by recombinant DNA technology for human therapeutic use. A brief overview of insulin production from both traditional mammalian pancreatic extraction and recombinant bacterial and yeast systems is presented, and detection techniques, including electrophoresis, are reviewed. Analytical systems for insulin separation are principally based on reversed-phase chromatography, which resolves the deamidation product(s) (desamido insulin) of insulin, proinsulin, and insulin. Process-scale separation is a multistep process and includes ion exchange, reversed-phase, and size exclusion chromatography. Advantages and/or disadvantages of various separation approaches, as described by the numerous literature references on insulin purification, are presented.
胰岛素是一种特性明确的肽，可通过重组DNA技术生产用于人类治疗。本文简要概述了从传统哺乳动物胰腺提取以及重组细菌和酵母系统生产胰岛素的情况，并对包括电泳在内的检测技术进行了综述。胰岛素分离分析系统主要基于反相色谱法，该方法可分离胰岛素、胰岛素原和胰岛素的脱酰胺产物（脱酰胺胰岛素）。生产规模的分离是一个多步骤过程，包括离子交换、反相和尺寸排阻色谱法。文中介绍了众多胰岛素纯化文献中描述的各种分离方法的优缺点。
