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用于尿素酶促水解的固体负载型脲酶和可溶性脲酶性能的比较研究。

A comparative study of the performance of solid supported and soluble urease for the enzymatic hydrolysis of urea.

作者信息

Demirel G, Akovali G, Tanyolac A, Hasirci N

机构信息

Department of Chemistry, Middle East Technical University, Ankara, Turkey.

出版信息

J Chem Technol Biotechnol. 1992;55(4):319-23. doi: 10.1002/jctb.280550403.

DOI:10.1002/jctb.280550403
PMID:1369049
Abstract

The performance of both free and solid supported urease for the enzymatic hydrolysis of urea was studied. Kinetic analysis of reaction rate data shows that the kinetic data were consistent with the proposed model. Statistical tests validated the model. Despite the deactivation of ureases, the immobilized enzyme could be the choice for a technological process aiming at the detoxification of blood in haemoperfusion columns.

摘要

研究了游离脲酶和固定化脲酶对尿素的酶促水解性能。反应速率数据的动力学分析表明,动力学数据与所提出的模型一致。统计检验验证了该模型。尽管脲酶会失活,但固定化酶可能是用于血液灌流柱中血液解毒工艺过程的选择。

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A comparative study of the performance of solid supported and soluble urease for the enzymatic hydrolysis of urea.用于尿素酶促水解的固体负载型脲酶和可溶性脲酶性能的比较研究。
J Chem Technol Biotechnol. 1992;55(4):319-23. doi: 10.1002/jctb.280550403.
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引用本文的文献

1
Kinetic studies of the urease-catalyzed hydrolysis of urea in a buffer-free system.在无缓冲体系中脲酶催化尿素水解的动力学研究。
Appl Biochem Biotechnol. 1994 Dec;49(3):217-40. doi: 10.1007/BF02783059.