Kobayashi H, Inokuchi N, Koyama T, Watanabe H, Iwama M, Ohgi K, Irie M
Department of Microbiology, College of Pharmacy, Nihon University, Chiba, Japan.
Biosci Biotechnol Biochem. 1992 Dec;56(12):2003-10. doi: 10.1271/bbb.56.2003.
The complete primary structure of a base non-specific and adenylic acid preferential RNase (RNase Le2) from the fruit bodies of Lentinus edodes was analyzed. The sequence was mostly determined by analysis of the peptides generated by V8 protease digestion and BrCN cleavage (including alpha-chymotryptic, and V8 protease digest of BrCN fragments). It consists of 239 amino acid residues. The molecular weight is 25831. The location of 10 half cystine residues were almost superimposable on those of known fungal RNases of the RNase T2 family. The sequence homologies between RNase Le2 and four known fungal RNases of the RNase T2 family, RNase T2, RNase M, RNase Trv, and RNase Rh, are 102, 103, 109, and 74, respectively. The homologous sequences are concentrated around the three histidines, which are supposed to form the active site of RNase T2 family RNases.
对香菇子实体中一种碱基非特异性且优先作用于腺苷酸的核糖核酸酶（核糖核酸酶Le2）的完整一级结构进行了分析。该序列主要通过分析V8蛋白酶消化和溴化氰裂解产生的肽段来确定（包括α-胰凝乳蛋白酶以及溴化氰片段的V8蛋白酶消化产物）。它由239个氨基酸残基组成。分子量为25831。10个半胱氨酸残基的位置与核糖核酸酶T2家族已知真菌核糖核酸酶的位置几乎重叠。核糖核酸酶Le2与核糖核酸酶T2家族的四种已知真菌核糖核酸酶，即核糖核酸酶T2、核糖核酸酶M、核糖核酸酶Trv和核糖核酸酶Rh的序列同源性分别为102、103、109和74。同源序列集中在三个组氨酸周围，这三个组氨酸被认为构成核糖核酸酶T2家族核糖核酸酶的活性位点。
