通过在组氨酸 -AH -琼脂糖上进行假生物亲和色谱法从人血浆的IgG -科恩II组分中分离和纯化IgG1和IgG2 。
Separation and purification of IgG1 and IgG2 from IgG-Cohn-II fraction of human plasma by pseudobioaffinity chromatography on histidyl-AH-sepharose.
作者信息
el-Kak A, Vijayalakshmi M A
机构信息
Département de Génie Biologique, Université de Technologie de Compiègne, France.
出版信息
Bioseparation. 1992;3(1):47-53.
PMID:1369217
Abstract
L-histidine coupled to aminohexyl-sepharose (H-AH) has been used as an affinity sorbent to separate IgG from human plasma. Two subclasses IgG1 and IgG2 were specifically bound to histidyl-AH-sepharose at pH 7.4 and eluted using 0.2 M and 1M NaCl. The specificity of the two subclasses were determined by immunoelectrophoresis. Quantitative determination of IgG1, IgG2 was carried out using radial immunodiffusion technique.
摘要
与氨基己基琼脂糖（H-AH）偶联的L-组氨酸已被用作亲和吸附剂，从人血浆中分离IgG。在pH 7.4条件下，IgG1和IgG2两个亚类特异性结合到组氨酸-AH-琼脂糖上，并用0.2M和1M氯化钠洗脱。通过免疫电泳确定这两个亚类的特异性。使用放射免疫扩散技术对IgG1、IgG2进行定量测定。
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