Pernia S D, Berry D L, Redin W R, Carney D H
Department of Human Biological Chemistry & Genetics, University of Texas Medical Branch, Galveston 77550.
SAAS Bull Biochem Biotechnol. 1990 Jan;3:8-12.
Our studies of alpha-thrombin as a growth factor have led to the development of a synthetic peptide (p508) that in vitro competes with thrombin for binding to high affinity receptors, and enhances mitogenic activity. To determine if this peptide could be used to accelerate wound closure in vivo, full thickness 6 mm dermal biopsy wounds on the dorsal skin of anesthetized rats were treated with p508 peptide, thrombin or PBS as control. At day 7, the p508 treated wound areas were 20% to 50% smaller than either thrombin or PBS treated wound sites. This suggests that p508 enhances aspects of wound healing, and avoids the normal in vivo regulatory mechanisms of intact thrombin.
我们对作为生长因子的α-凝血酶的研究，促使我们开发出一种合成肽（p508），该肽在体外可与凝血酶竞争结合高亲和力受体，并增强促有丝分裂活性。为了确定这种肽是否可用于加速体内伤口愈合，将麻醉大鼠背部皮肤的全层6毫米皮肤活检伤口用p508肽、凝血酶或作为对照的PBS进行处理。在第7天，用p508处理的伤口面积比用凝血酶或PBS处理的伤口部位小20%至50%。这表明p508可增强伤口愈合的各个方面，并避开了完整凝血酶正常的体内调节机制。
