Pata J, Kirkegaard K
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347.
SAAS Bull Biochem Biotechnol. 1991 Jan;4:20-1.
We are developing an in vitro system for poliovirus RNA recombination. In this system, two mutant RNAs are replicated with poliovirus RNA-dependent RNA polymerase. Recombination will produce RNAs containing neither mutation and will be the only progeny RNAs that are infectious. We will use this system to determine what proteins and reaction conditions are required for recombination and to study the details of the mechanism of recombination.
我们正在开发一种用于脊髓灰质炎病毒RNA重组的体外系统。在这个系统中，两种突变RNA由脊髓灰质炎病毒RNA依赖性RNA聚合酶进行复制。重组将产生既不包含任何一种突变的RNA，并且将是唯一具有感染性的子代RNA。我们将利用这个系统来确定重组需要哪些蛋白质和反应条件，并研究重组机制的细节。
