Vidal M, Cairó J, Mateu M G, Villaverde A
Institut de Biologia Fonamental, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Appl Microbiol Biotechnol. 1991 Sep;35(6):788-92. doi: 10.1007/BF00169896.
The VP1 gene of foot-and-mouth disease virus (serotype C1) has been cloned in Escherichia coli Clts cells, under the control of the bacteriophage lambda pL promoter. The expressed VP1 protein was complete and non-fused, and its molecular weight was indistinguishable from that of the VP1 obtained from virions. Cells harbouring the recombinant vectors exhibited symptoms of plasmid instability and toxicity and died in a few weeks even when never exposed to inducing conditions. A new plasmid clone in which a segment of the VP1 gene was fused with contiguous genes of the viral genome was very stable. The expressed partial VP1 protein contains the two major immunogenic domains of the virion. This system can be used as a tool to design an immunogenic VP1, and to explore possible synthetic vaccines against foot-and-mouth disease.
口蹄疫病毒（C1血清型）的VP1基因已在大肠杆菌Clts细胞中克隆，受噬菌体λ pL启动子控制。表达的VP1蛋白完整且未融合，其分子量与从病毒粒子获得的VP1无法区分。携带重组载体的细胞表现出质粒不稳定性和毒性症状，即使从未暴露于诱导条件下，几周内也会死亡。一个新的质粒克隆非常稳定，其中VP1基因的一段与病毒基因组的相邻基因融合。表达的部分VP1蛋白包含病毒粒子的两个主要免疫原性结构域。该系统可作为设计免疫原性VP1以及探索针对口蹄疫的可能合成疫苗的工具。
