Küpper H, Keller W, Kurz C, Forss S, Schaller H, Franze R, Strohmaier K, Marquardt O, Zaslavsky V G, Hofschneider P H
Nature. 1981 Feb 12;289(5798):555-9. doi: 10.1038/289555a0.
Double-stranded DNA copies of the single-stranded genomic RNA of foot and mouth disease virus have been cloned into the Escherichia coli plasmid pBR322. A restriction map of the viral genome was established and aligned with the biochemical map of foot and mouth disease virus. The coding sequence for structural protein VP1, the major antigen of the virus, was identified and inserted into a plasmid vector where the expression of this sequence is under control of the phage lambda PL promoter. In an appropriate host the synthesis of antigenic polypeptide can be demonstrated by radioimmunoassay.
口蹄疫病毒单链基因组RNA的双链DNA拷贝已被克隆到大肠杆菌质粒pBR322中。建立了病毒基因组的限制性图谱，并与口蹄疫病毒的生化图谱进行了比对。确定了病毒主要抗原结构蛋白VP1的编码序列，并将其插入到一个质粒载体中，该序列的表达受噬菌体λ PL启动子的控制。在合适的宿主中，可通过放射免疫测定法证明抗原多肽的合成。
