S-Zephyr, a new high performance ion exchange chromatography column matrix.

S-Zephyr, a new column material for high performance cation exchange chromatography of proteins, is compared with Mono-S. The comparison is based on a retentivity study, a model separation of an artificial protein mixture, a sample load capacity experiment and the development of the separation performance at a column overload situation. S-Zephyr is found to be a good matrix for cation exchange chromatography, for analytical separations as well as for small and large scale protein purification applications.