Rongen R M, Adler C M, Scouten W H
Department of Chemistry, Baylor University, Waco, TX 767698-7348.
Bioseparation. 1991;2(5):297-308.
S-Zephyr, a new column material for high performance cation exchange chromatography of proteins, is compared with Mono-S. The comparison is based on a retentivity study, a model separation of an artificial protein mixture, a sample load capacity experiment and the development of the separation performance at a column overload situation. S-Zephyr is found to be a good matrix for cation exchange chromatography, for analytical separations as well as for small and large scale protein purification applications.
S-Zephyr是一种用于蛋白质高效阳离子交换色谱的新型柱材料，将其与Mono-S进行了比较。该比较基于保留性研究、人工蛋白质混合物的模型分离、样品负载量实验以及柱过载情况下分离性能的发展。结果发现，S-Zephyr是阳离子交换色谱的良好基质，适用于分析分离以及小规模和大规模蛋白质纯化应用。
