de Lorenzo V
Centro de Investigaciones Biológicas-CSIC, Madrid, Spain.
Curr Opin Biotechnol. 1992 Jun;3(3):227-31. doi: 10.1016/0958-1669(92)90097-3.
Environmental applications of genetically engineered microorganisms are currently hampered not only by legal regulations restricting their release, but also by the frequent dearth of adequate genetic tools for their construction in the laboratory. Recent approaches to strain development include the use of non-antibiotic markers as selection determinants, the use of transposon-vectors for the permanent acquisition of recombinant genes, and the utilization of expression devices based on promoters from promiscuous plasmids and biodegradative pathway genes.
目前，基因工程微生物在环境中的应用不仅受到限制其释放的法律法规的阻碍，还受到实验室中构建它们时常用的合适基因工具匮乏的影响。最近的菌株开发方法包括使用非抗生素标记作为选择决定因素，使用转座子载体永久获取重组基因，以及利用基于来自滥交质粒的启动子和生物降解途径基因的表达装置。
