牙龈卟啉单胞菌W83主要硫醇激活半胱氨酸蛋白酶特异性单克隆抗体的产生及特性分析

Generation and characterization of a monoclonal antibody specific for the major thiol-activated cysteine proteinase of Porphyromonas gingivalis W83.

作者信息

Wallace A, Rigg G, Hyman S C, James R, Roberts I S

机构信息

Department of Microbiology, University of Leicester, UK.

出版信息

Lett Appl Microbiol. 1992 Nov;15(5):202-6. doi: 10.1111/j.1472-765x.1992.tb00763.x.

DOI:10.1111/j.1472-765x.1992.tb00763.x

PMID:1369405

Abstract

An IgM monoclonal antibody specified for the thiol-activated proteinase of the oral pathogen Porphyromonas gingivalis W83 was generated. The antibody reacted with a single protein of approximate molecular mass 43 kDa in outer membrane preparations of P. gingivalis. Immuno-electron microscopy using the monoclonal antibody and immunogold labelling confirmed the cell surface location of the thiol-activated proteinase. The monoclonal antibody failed to detect any proteins in Western blot analysis of other closely related oral bacteria. The specificity of this monoclonal antibody to the thiol-activated proteinase of P. gingivalis should allow its use as a diagnostic tool for the rapid enumeration of P. gingivalis in clinical samples.

摘要

制备了一种针对口腔病原体牙龈卟啉单胞菌W83巯基激活蛋白酶的IgM单克隆抗体。该抗体与牙龈卟啉单胞菌外膜制剂中一条分子量约为43 kDa的单一蛋白质发生反应。使用该单克隆抗体和免疫金标记的免疫电子显微镜证实了巯基激活蛋白酶的细胞表面定位。在对其他密切相关口腔细菌的蛋白质印迹分析中，该单克隆抗体未能检测到任何蛋白质。这种针对牙龈卟啉单胞菌巯基激活蛋白酶的单克隆抗体的特异性使其可作为一种诊断工具，用于临床样本中牙龈卟啉单胞菌的快速计数。