Grayburn W S, Collins G B, Hildebrand D F
Department of Agronomy, University of Kentucky, Lexington 40546-0091.
Biotechnology (N Y). 1992 Jun;10(6):675-8. doi: 10.1038/nbt0692-675.
Nicotiana tabacum tissue was transformed with a rat stearyl-CoA desaturase gene. Gas chromatographic analysis showed an increase in monounsaturated 16 and 18 carbon fatty acids in selected transformed calli and leaves. Fractionation of lipid classes indicated that palmitoleic acid was found in the phosphatidylcholine fraction of desaturase-transformed leaves, but not in leaves transformed with vector sequences. Plant transformation was verified by polymerase chain reaction (PCR) amplification of total leaf DNA.
用大鼠硬脂酰辅酶A去饱和酶基因转化烟草组织。气相色谱分析表明，在选定的转化愈伤组织和叶片中，单不饱和的16碳和18碳脂肪酸有所增加。脂质类别的分级分离表明，棕榈油酸存在于去饱和酶转化叶片的磷脂酰胆碱部分，而在载体序列转化的叶片中则未发现。通过对叶片总DNA进行聚合酶链反应（PCR）扩增来验证植物转化。
