ESTABROOK R W, HOLOWINSKY A
J Biophys Biochem Cytol. 1961 Jan;9(1):19-28. doi: 10.1083/jcb.9.1.19.
(1) The mathematical calculations relating spectrophotometric data with the data of Allard et al. (4, 5) on mitochondrial counts, is presented. Such a calculation indicates that an "average mitochondrion" from rat liver would contain about 17,000 molecules of each cytochrome pigment. (2) Hematocrit determinations relating respiratory pigment content for mitochondria isolated from a variety of tissues have been presented, showing a fivefold variability depending upon the source of the mitochondria. (3) Speculations on the organization of the respiratory enzymes associated with the membrane structure of the mitochondria are discussed.
(1) 给出了将分光光度数据与阿拉德等人关于线粒体计数的数据(4, 5)相关联的数学计算。这样的计算表明,来自大鼠肝脏的“平均线粒体”每种细胞色素色素大约含有17,000个分子。(2) 给出了从多种组织分离的线粒体的血细胞比容测定与呼吸色素含量的关系,表明根据线粒体的来源有五倍的变化。(3) 讨论了关于与线粒体膜结构相关的呼吸酶组织的推测。