FAHEY J L
J Exp Med. 1961 Sep 1;114(3):385-98. doi: 10.1084/jem.114.3.385.
The serum myeloma proteins associated with 20 mouse plasma cell tumors in C(3)H or BALB/c mice that had proved transplantable were characterized by electrophoretic and immunochemical techniques. Although the myeloma proteins ranged in electrophoretic mobility from gamma to alpha globulins, they could be divided into two groups, the gamma type and the beta type myeloma globulins, on the basis of characteristic immunochemical properties. Gamma type myeloma proteins (5563, MPC-11) showed a close immunochemical relationship to normal mouse gamma globulins. Eighteen beta type mouse myeloma proteins migrated as beta or alpha globulins on zone electrophoresis. These proteins shared common antigenic features which permitted their recognition, separate from gamma myeloma proteins. The beta type myeloma proteins were shown to be related to a beta globulin component present in normal serum. Strain differences were observed for the normal beta globulin component believed to be formed in plasma cells. The proteins formed in mouse plasma cells were found to be antigenically complex. Shared antigenic determinants as well as distinctive antigenic determinants were detected when representative myeloma proteins were purified and compared by the Ouchterlony double diffusion technique. The myeloma proteins associated with each of the transplantable plasma cell tumors in mice are regarded as distinctive and characteristic products of plasma cell metabolism. The variety of myeloma globulins was similar for plasma cell tumors arising in C(3)H as well as in BALB/c mice, indicating that differences in mouse strains would not account for the differences among the myeloma globulins. These differences, however, may be due to differences among the normal plasma cells from which the malignant cells are derived. If this is so, the variety of myeloma globulins reflect the variety of plasma cells present normally.
运用电泳和免疫化学技术，对20种已证实可移植的C(3)H或BALB/c小鼠浆细胞瘤所产生的血清骨髓瘤蛋白进行了特性分析。尽管骨髓瘤蛋白的电泳迁移率范围从γ球蛋白到α球蛋白，但根据其独特的免疫化学特性，可将它们分为两组，即γ型和β型骨髓瘤球蛋白。γ型骨髓瘤蛋白（5563、MPC - 11）与正常小鼠γ球蛋白呈现出密切的免疫化学关系。18种β型小鼠骨髓瘤蛋白在区带电泳中迁移为β球蛋白或α球蛋白。这些蛋白具有共同的抗原特征，从而得以与γ骨髓瘤蛋白区分开来。已证明β型骨髓瘤蛋白与正常血清中存在的一种β球蛋白成分相关。据信在浆细胞中形成的正常β球蛋白成分存在品系差异。发现小鼠浆细胞中形成的蛋白在抗原方面较为复杂。当通过Ouchterlony双向扩散技术对代表性骨髓瘤蛋白进行纯化和比较时，检测到了共同的抗原决定簇以及独特的抗原决定簇。与小鼠中每种可移植浆细胞瘤相关的骨髓瘤蛋白被视为浆细胞代谢的独特且具有特征性的产物。C(3)H小鼠和BALB/c小鼠中产生的浆细胞瘤的骨髓瘤球蛋白种类相似，这表明小鼠品系的差异并不能解释骨髓瘤球蛋白之间的差异。然而，这些差异可能是由于恶性细胞所源自的正常浆细胞之间的差异所致。如果是这样，骨髓瘤球蛋白的多样性反映了正常情况下存在的浆细胞的多样性。
