MIGITA S, PUTNAM F W
J Exp Med. 1963 Jan 1;117(1):81-104. doi: 10.1084/jem.117.1.81.
By means of immunodiffusion and immunoelectrophoresis study has been made of antigenic relationships of Bence Jones proteins, and the three classes of normal and pathological immunoglobulins, 7S gamma, beta(2A), and beta(2M). All thirty-nine Bence Jones proteins studied could be classified into either one of two distinct antigenic types, A or B. Both types are related to the immunoelectrophoretically slow (S) fragment of a papain digest of normal gamma-globulin; B is related more closely than A, but neither has antigenic determinants in common with the fast (F) fragment. The 7S gamma myeloma globulins were either immunological type I or II. The papain digests of these proteins produced the S and F precipitin lines in immunoelectrophoresis but multiple bands in starch gel electrophoresis, especially in the F region. The S fraction of type I myeloma globulins is antigenically similar to Bence Jones protein of type B, and the S component of type II myeloma globulins has antigenic determinants in common with type A Bence Jones protein. Correspondingly, myeloma patients with type I globulins and proteinuria usually excrete type B Bence Jones proteins, whereas patients with type II excrete type A proteins. The F fragment is the part common to normal 7S gamma-globulin and types I and II myeloma globulins but is absent in beta(2A) and beta(2M) pathological globulins and in both types of Bence Jones proteins. Papain digests of beta(2A) myeloma globulins produced a single precipitin line in immunoelectrophoresis. beta(2A) myeloma globulins appeared to have two antigenic units, one in common with type B Bence Jones protein and normal gamma-globulin, and another specific to beta(2A). The beta(2A) myeloma patients excreted type B Bence Jones protein. The papain digest of a macroglobulin produced two precipitin lines, the faster of which had antigenic determinants in common with type B Bence Jones protein, the slower seemed specific for the macroglobulin. Five serum micromolecular globulins proved to be either type A or B Bence Jones proteins. From the above results, an antigenic map was constructed showing which determinants are shared and which are specific for normal 7S gamma-globulin, types I and II myeloma globulins, beta(2A) myeloma globulins, a macroglobulin, and types A and B Bence Jones proteins.
通过免疫扩散和免疫电泳法，对本-周蛋白与三类正常及病理性免疫球蛋白（7Sγ、β(2A)和β(2M)）的抗原关系进行了研究。所研究的39种本-周蛋白均可分为两种不同的抗原类型，即A或B。这两种类型均与正常γ球蛋白木瓜蛋白酶消化产物免疫电泳慢（S）片段相关；B型与该片段的相关性比A型更为密切，但二者均与快（F）片段无共同抗原决定簇。7Sγ骨髓瘤球蛋白为免疫I型或II型。这些蛋白的木瓜蛋白酶消化产物在免疫电泳中产生S和F沉淀线，但在淀粉凝胶电泳中出现多条带，尤其是在F区。I型骨髓瘤球蛋白的S组分在抗原性上与B型本-周蛋白相似，II型骨髓瘤球蛋白的S组分与A型本-周蛋白有共同抗原决定簇。相应地，I型球蛋白和蛋白尿的骨髓瘤患者通常排泄B型本-周蛋白，而II型患者排泄A型蛋白。F片段是正常7Sγ球蛋白以及I型和II型骨髓瘤球蛋白共有的部分，但在β(2A)和β(2M)病理性球蛋白以及两种类型的本-周蛋白中均不存在。β(2A)骨髓瘤球蛋白的木瓜蛋白酶消化产物在免疫电泳中产生一条单一沉淀线。β(2A)骨髓瘤球蛋白似乎有两个抗原单位，一个与B型本-周蛋白和正常γ球蛋白共有，另一个是β(2A)特有的。β(2A)骨髓瘤患者排泄B型本-周蛋白。一种巨球蛋白的木瓜蛋白酶消化产物产生两条沉淀线，其中较快的一条与B型本-周蛋白有共同抗原决定簇，较慢的一条似乎是该巨球蛋白特有的。5种血清微分子球蛋白被证明为A型或B型本-周蛋白。根据上述结果构建了一张抗原图谱，显示了正常7Sγ球蛋白、I型和II型骨髓瘤球蛋白、β(2A)骨髓瘤球蛋白、一种巨球蛋白以及A型和B型本-周蛋白共有的和特有的决定簇。
