FELL H B, THOMAS L
J Exp Med. 1961 Sep 1;114(3):343-62. doi: 10.1084/jem.114.3.343.
The effect of hydrocortisone has been studied in organ cultures of the cartilaginous long bone rudiments from 7-day chick embryos and of the well ossified limb bones from late fetal mice. In the chick rudiments, which grow rapidly in culture, the growth rate was much reduced by hydrocortisone, less intercellular material was formed, and the hypertrophic cells of the shaft were much smaller than in the controls in normal medium. In the late fetal mouse bones, which grow very little in culture, hydrocortisone had no obvious effect on growth but arrested resorption of the cartilage. These effects resemble those described by others in the skeleton of animals treated with cortisone or hydrocortisone. The influence of hydrocortisone on the response of the chick and mouse explants to excess vitamin A was investigated. In the presence of excess vitamin A, cartilage (chick, mouse) and bone (mouse) rapidly disintegrated, but when hydrocortisone also was added to the medium, this dissolution of the intercellular material was much retarded, though not suppressed. The retardative action of hydrocortisone on the changes produced by excess vitamin A in skeletal tissue in culture, contrasts sharply with the strongly additive effect of the two agents on the skeleton in the intact animal (Selye, 1958). It is suggested that this discrepancy between the results obtained in vitro and in vivo is probably due to systemic factors that operate in the body but are eliminated in organ cultures.
已经在来自7日龄鸡胚胎的软骨长骨原基以及来自晚期胎鼠的充分骨化的四肢骨骼的器官培养物中研究了氢化可的松的作用。在培养中生长迅速的鸡原基中,氢化可的松使生长速率大大降低,形成的细胞间物质减少,并且骨干的肥大细胞比正常培养基中的对照小得多。在培养中生长很少的晚期胎鼠骨骼中,氢化可的松对生长没有明显影响,但阻止了软骨的吸收。这些作用类似于其他用可的松或氢化可的松处理的动物骨骼中所描述的作用。研究了氢化可的松对鸡和小鼠外植体对过量维生素A反应的影响。在存在过量维生素A的情况下,软骨(鸡、小鼠)和骨骼(小鼠)迅速分解,但当培养基中也加入氢化可的松时,这种细胞间物质的溶解被大大延迟,尽管没有被抑制。氢化可的松对培养的骨骼组织中过量维生素A产生的变化的延迟作用,与这两种物质在完整动物体内对骨骼的强烈相加作用形成鲜明对比(Selye,1958)。有人认为,体外和体内获得的结果之间的这种差异可能是由于体内起作用但在器官培养中被消除的全身因素。