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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Dingle J T, Fell H B, Lucy J A

Biochem J. 1966 Jan;98(1):173-81. doi: 10.1042/bj0980173.

The action of excess of retinol on chick limb-bone rudiments cultured in chemically defined media has been investigated. 2. After 6 days in vitro, the hexosamine and hydroxyproline contents of the retinol-treated rudiments were much less than those of their paired controls. 3. Synthesis of these compounds, however, was not correspondingly decreased. 4. The control rudiments released hexosamine- and hydroxyproline-containing materials into the culture medium; a greater proportion of the hexosamine and hydroxyproline synthesized was liberated from the retinol-treated than from the control rudiments. 5. Although hydrocortisone in physiological concentrations prevented excessive hydration of the rudiments in culture, it did not significantly inhibit the changes induced by excess of retinol.

研究了过量视黄醇对在化学成分明确的培养基中培养的鸡胚肢骨原基的作用。2. 体外培养6天后，视黄醇处理的原基中的己糖胺和羟脯氨酸含量远低于其配对对照。3. 然而，这些化合物的合成并未相应减少。4. 对照原基将含己糖胺和羟脯氨酸的物质释放到培养基中；视黄醇处理的原基中合成的己糖胺和羟脯氨酸比对照原基释放出的比例更大。5. 尽管生理浓度的氢化可的松可防止培养中原基过度水化，但它并未显著抑制过量视黄醇引起的变化。

Dingle J T, Fell H B, Lucy J A

Biochem J. 1966 Jan;98(1):173-81. doi: 10.1042/bj0980173.

The action of excess of retinol on chick limb-bone rudiments cultured in chemically defined media has been investigated. 2. After 6 days in vitro, the hexosamine and hydroxyproline contents of the retinol-treated rudiments were much less than those of their paired controls. 3. Synthesis of these compounds, however, was not correspondingly decreased. 4. The control rudiments released hexosamine- and hydroxyproline-containing materials into the culture medium; a greater proportion of the hexosamine and hydroxyproline synthesized was liberated from the retinol-treated than from the control rudiments. 5. Although hydrocortisone in physiological concentrations prevented excessive hydration of the rudiments in culture, it did not significantly inhibit the changes induced by excess of retinol.

研究了过量视黄醇对在化学成分明确的培养基中培养的鸡胚肢骨原基的作用。2. 体外培养6天后，视黄醇处理的原基中的己糖胺和羟脯氨酸含量远低于其配对对照。3. 然而，这些化合物的合成并未相应减少。4. 对照原基将含己糖胺和羟脯氨酸的物质释放到培养基中；视黄醇处理的原基中合成的己糖胺和羟脯氨酸比对照原基释放出的比例更大。5. 尽管生理浓度的氢化可的松可防止培养中原基过度水化，但它并未显著抑制过量视黄醇引起的变化。