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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Katsumata Y, Tanaka F, Hagihara M, Yagi K, Yamanaka N

Biochim Biophys Acta. 1976 Dec 2;455(2):399-411. doi: 10.1016/0005-2736(76)90314-x.

Sarcoplasmic reticulum membranes were labelled with 1-dimethylaminonaphtalene-5-sulfonyl chloride (DnsCl). Analyses of the dansylated membranes demonstrated that the most of the dye was associated with ATPase (ATP phosphohydrolase, EC 3.6.1.3) and phosphatidylethanolamine in the membranes. 2. Dansylation of the membranes could be performed without significant decrease in the ATPase activity. 3. Partial differentiation of fluorescence of Dns-phosphatidylethanolamine from that of Dns-ATPase could be achieved by changing excitation wavelength; Dns-ATPase emmitted in the shorter wavelength region, while Dns-phosphatidylethanolamine emmitted in the longer wavelength region. 4. Fluorescence polarization of the dye bound to the membranes indicated that both the ATPase and phosphatidylethanolamine were strongly immobilized in the membranes, while the ratio of freely rotating dye to the "frozen" dye bound to the ATPase was larger than that bound to the phosphatide.

肌浆网膜用1-二甲氨基萘-5-磺酰氯（DnsCl）进行标记。对丹磺酰化膜的分析表明，大部分染料与膜中的ATP酶（ATP磷酸水解酶，EC 3.6.1.3）和磷脂酰乙醇胺相关。2. 膜的丹磺酰化可以在不显著降低ATP酶活性的情况下进行。3. 通过改变激发波长，可以实现丹磺酰化磷脂酰乙醇胺与丹磺酰化ATP酶荧光的部分区分；丹磺酰化ATP酶在较短波长区域发射荧光，而丹磺酰化磷脂酰乙醇胺在较长波长区域发射荧光。4. 与膜结合的染料的荧光偏振表明，ATP酶和磷脂酰乙醇胺在膜中都被强烈固定，而与ATP酶结合的自由旋转染料与“固定”染料的比例大于与磷脂结合的比例。

Katsumata Y, Tanaka F, Hagihara M, Yagi K, Yamanaka N

Biochim Biophys Acta. 1976 Dec 2;455(2):399-411. doi: 10.1016/0005-2736(76)90314-x.

Sarcoplasmic reticulum membranes were labelled with 1-dimethylaminonaphtalene-5-sulfonyl chloride (DnsCl). Analyses of the dansylated membranes demonstrated that the most of the dye was associated with ATPase (ATP phosphohydrolase, EC 3.6.1.3) and phosphatidylethanolamine in the membranes. 2. Dansylation of the membranes could be performed without significant decrease in the ATPase activity. 3. Partial differentiation of fluorescence of Dns-phosphatidylethanolamine from that of Dns-ATPase could be achieved by changing excitation wavelength; Dns-ATPase emmitted in the shorter wavelength region, while Dns-phosphatidylethanolamine emmitted in the longer wavelength region. 4. Fluorescence polarization of the dye bound to the membranes indicated that both the ATPase and phosphatidylethanolamine were strongly immobilized in the membranes, while the ratio of freely rotating dye to the "frozen" dye bound to the ATPase was larger than that bound to the phosphatide.

肌浆网膜用1-二甲氨基萘-5-磺酰氯（DnsCl）进行标记。对丹磺酰化膜的分析表明，大部分染料与膜中的ATP酶（ATP磷酸水解酶，EC 3.6.1.3）和磷脂酰乙醇胺相关。2. 膜的丹磺酰化可以在不显著降低ATP酶活性的情况下进行。3. 通过改变激发波长，可以实现丹磺酰化磷脂酰乙醇胺与丹磺酰化ATP酶荧光的部分区分；丹磺酰化ATP酶在较短波长区域发射荧光，而丹磺酰化磷脂酰乙醇胺在较长波长区域发射荧光。4. 与膜结合的染料的荧光偏振表明，ATP酶和磷脂酰乙醇胺在膜中都被强烈固定，而与ATP酶结合的自由旋转染料与“固定”染料的比例大于与磷脂结合的比例。