Tokiwa H, Horikawa K, Sera N
Department of Health Science, Fukuoka Environmental Research Center, Japan.
Mutat Res. 1992 Jan-Mar;276(1-2):139-44. doi: 10.1016/0165-1110(92)90063-f.
The mutagenicity of SRM 1649 and 1650 was tested in the presence of rat liver S9 mix which was induced by polychlorinated biphenyl (PCB) or by the combination of phenobarbital and 5,6-benzoflavone. The S9 mix induced by PCB activated benzo[a]pyrene strongly. The S9 mix induced by phenobarbital-5,6-benzoflavone activated the complex mixtures to approximately the same extent as that induced by PCB. This finding indicates that phenobarbital-5,6-benzoflavone instead of PCB may be suitable as an inducer under some conditions. The preincubation procedure for the mutagenicity test was performed by preincubating the test compound, S9 mix and bacteria for 20 min in a water bath. This procedure was as effective as the plate incorporation test.
在由多氯联苯（PCB）或苯巴比妥与5,6 - 苯并黄酮组合诱导产生的大鼠肝脏S9混合液存在的情况下，对SRM 1649和1650的致突变性进行了测试。由PCB诱导产生的S9混合液能强烈激活苯并[a]芘。由苯巴比妥 - 5,6 - 苯并黄酮诱导产生的S9混合液对复杂混合物的激活程度与由PCB诱导产生的S9混合液大致相同。这一发现表明，在某些条件下，苯巴比妥 - 5,6 - 苯并黄酮而非PCB可能适合作为诱导剂。致突变性测试的预孵育程序是将测试化合物、S9混合液和细菌在水浴中预孵育20分钟。该程序与平板掺入试验同样有效。
