可见光对吖啶橙-DNA复合物的损伤。
Damage by visible light to the acridine orange--DNA complex.
作者信息
FREIFELDER D, DAVISON P F, GEIDUSCHEK E P
出版信息
Biophys J. 1961 May;1(5):389-400. doi: 10.1016/s0006-3495(61)86897-5.
Abstract
Salmon DNA has been irradiated with visible light in the presence of acridine orange. If the dye is bound to the DNA, there results: (a) a decrease in sedimentation coefficient, (b) a lowering of viscosity, and (c) a decrease in the thermal denaturation temperature. CsCl banding experiments show that the first two effects reflect depolymerization of the DNA. Depolymerization apparently occurs by single-strand scission although some double-strand scission is not excluded. The destabilization of secondary structure results probably from chemical attack on the components of the individual strands.
摘要
在吖啶橙存在的情况下，鲑鱼脱氧核糖核酸（DNA）已用可见光进行了照射。如果该染料与DNA结合，会产生以下结果：（a）沉降系数降低，（b）粘度下降，以及（c）热变性温度降低。氯化铯密度梯度离心实验表明，前两种效应反映了DNA的解聚。解聚显然是通过单链断裂发生的，尽管不排除一些双链断裂的情况。二级结构的不稳定可能是由于对单链成分的化学攻击所致。
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