Nikonowicz E P, Pardi A
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215.
Nature. 1992 Jan 9;355(6356):184-6. doi: 10.1038/355184a0.
Multidimensional heteronuclear NMR has revolutionized solution structure determinations of proteins. But this technique has not been applied to nucleic acids because of difficulties in the synthesis of isotopically (13C and/or 15N) labelled molecules. Here we report the application of three-dimensional heteronuclear NMR to the study of a uniformly 13C/15N or 15N-labelled RNA duplex of defined sequence. These experiments simplify resonance assignment and the analysis of proton-proton nuclear Overhauser effects (and therefore distance information) in the molecule. Our results show that it is now possible to determine the structures of larger and more complex RNAs using multidimensional heteronuclear NMR.
多维异核核磁共振技术彻底改变了蛋白质溶液结构的测定方法。但由于难以合成同位素（13C和/或15N）标记的分子，该技术尚未应用于核酸研究。在此，我们报告了三维异核核磁共振技术在研究具有特定序列的均匀13C/15N或15N标记的RNA双链体中的应用。这些实验简化了分子中共振归属以及质子-质子核Overhauser效应（进而距离信息）的分析。我们的结果表明，现在使用多维异核核磁共振技术确定更大、更复杂RNA的结构成为可能。
