Whitaker J N, Kirk K A, Herman P K, Zhou S R, Goodin R R, Moscarello M A, Wood D D
Department of Neurology, University of Alabama, Birmingham 35294.
J Neuroimmunol. 1992 Feb;36(2-3):135-46. doi: 10.1016/0165-5728(92)90045-m.
An immunochemical analysis was conducted to compare the C1 isomer of human myelin basic protein (MBP) with the newly described and less cationic, citrullinated isomer of MBP referred to as C8. Ten polyclonal antisera directed at multiple epitopes or restricted regions of MBP were used in radioimmunoassays to examine MBP-C1 and MBP-C8. Antisera reactive with MBP peptide 1-14 clearly distinguished MBP-C1 from MBP-C8. Antisera to human MBP peptides 10-19 and 90-170, but not to MBP peptide 69-89, showed modest differences between MBP-C1 and MBP-C8. The MBP-C8s from multiple sclerosis (MS) and non-MS brain reacted essentially the same. With murine monoclonal antibodies and enzyme-linked immunosorbent assay (ELISA), differences between MBP-C8 and other isomers were shown for anti-MBP 10-19 but not for anti-MBP 1-9 or anti-MBP 80-89. These findings imply differences in sequence or conformation in the structure of MBP-C7 compared to MBP-C1, most notably near the amino terminus.
进行了一项免疫化学分析，以比较人髓鞘碱性蛋白（MBP）的C1异构体与新描述的、阳离子性较弱的MBP瓜氨酸化异构体（称为C8）。在放射免疫分析中使用了针对MBP多个表位或受限区域的10种多克隆抗血清，以检测MBP-C1和MBP-C8。与MBP肽1-14反应的抗血清能清楚地区分MBP-C1和MBP-C8。针对人MBP肽10-19和90-170的抗血清（但不是针对MBP肽69-89的抗血清）显示MBP-C1和MBP-C8之间存在适度差异。来自多发性硬化症（MS）和非MS脑的MBP-C8反应基本相同。使用鼠单克隆抗体和酶联免疫吸附测定（ELISA），抗MBP 10-19显示出MBP-C8与其他异构体之间的差异，但抗MBP 1-9或抗MBP 80-89则未显示出差异。这些发现表明，与MBP-C1相比，MBP-C7的结构在序列或构象上存在差异，最明显的是在氨基末端附近。
