Régely K, Mándi Y, Béládi I
Mikrobiológiai Intézet, Szent-Györgyi A. Orvostudományi Egyetem, Szeged.
Orv Hetil. 1992 Jan 12;133(2):77-81.
The natural killing of K 562 cells by whole blood from normal subjects was comparable with that shown by separated mononuclear cells. In order to establish the conditions for a reliable natural killer assay by using very small numbers of effector cells in whole blood, the isotope uptake of target cells was increased by a modified labelling method, which permitted the use of fewer target cells in the assay. The natural cytotoxicity of whole blood was augmented by interferon to the same extent as observed with separated mononuclear cells. The chemiluminescence of granulocytes in whole blood comparable with that of separated granulocytes. Taken together, these methods are considerably less tedious than the conventional methods, technique is also economical, and the results may reflect in vivo cytolytic processes much better.
正常受试者全血对K562细胞的自然杀伤作用与分离的单核细胞所表现出的作用相当。为了建立一种通过在全血中使用极少量效应细胞进行可靠的自然杀伤试验的条件，采用一种改良的标记方法增加了靶细胞的同位素摄取，这使得试验中可以使用更少的靶细胞。全血的自然细胞毒性被干扰素增强到与分离的单核细胞所观察到的相同程度。全血中粒细胞的化学发光与分离的粒细胞相当。综上所述，这些方法比传统方法繁琐程度大大降低，技术也经济，并且结果可能更好地反映体内细胞溶解过程。
