Morrish D W, Honoré L H, Bhardwaj D
Department of Medicine, University of Alberta, Edmonton, Canada.
Am J Obstet Gynecol. 1992 Jan;166(1 Pt 1):160-6. doi: 10.1016/0002-9378(92)91853-3.
The null hypothesis is that partial hydatidiform moles have normal differentiated function (human chorionic gonadotropin and human placental lactogen secretion) in response to epidermal growth factor and 8-bromo-cyclic adenosine monophosphate.
Two complete moles, 10 partial hydatidiform moles, and 19 normal first-trimester placentas in monolayer culture were exposed to 10 ng/ml epidermal growth factor, 1 mmol/L 8-bromo-cyclic adenosine monophosphate plus 1 mmol/L theophylline, or control. Human chorionic gonadotropin and human placental lactogen secretion was measured. Frequency of response to stimuli was compared by chi 2 analysis, and hormone secretion was compared by analysis of variance.
Partial moles demonstrated reduced frequencies of response of human chorionic gonadotropin and human placental lactogen to epidermal growth factor (partial moles 2/8 and 2/8, respectively; normal placentas 16/19 and 7/18, respectively; p less than 0.025) and of human chorionic gonadotropin to 8-bromo-cyclic adenosine monophosphate (partial moles 3/5, normal placentas 13/16; p less than 0.005).
Partial hydatidiform moles demonstrate impaired human chorionic gonadotropin and human placental lactogen secretory responsiveness to epidermal growth factor and cyclic nucleotides in comparison with normal first-trimester trophoblast.
无效假设为部分性葡萄胎对表皮生长因子和8-溴环磷酸腺苷具有正常的分化功能（人绒毛膜促性腺激素和人胎盘催乳素分泌）。
将2例完全性葡萄胎、10例部分性葡萄胎和19例孕早期正常胎盘进行单层培养，分别暴露于10 ng/ml表皮生长因子、1 mmol/L 8-溴环磷酸腺苷加1 mmol/L氨茶碱或对照物中。检测人绒毛膜促性腺激素和人胎盘催乳素的分泌情况。通过卡方分析比较对刺激的反应频率，通过方差分析比较激素分泌情况。
部分性葡萄胎中人绒毛膜促性腺激素和人胎盘催乳素对表皮生长因子的反应频率降低（部分性葡萄胎分别为2/8和2/8；正常胎盘分别为16/19和7/18；p<0.025），人绒毛膜促性腺激素对8-溴环磷酸腺苷的反应频率也降低（部分性葡萄胎为3/5，正常胎盘为13/16；p<0.005）。
与孕早期正常滋养层相比，部分性葡萄胎中人绒毛膜促性腺激素和人胎盘催乳素对表皮生长因子和环核苷酸的分泌反应受损。
