肝细胞生长因子是肾上皮细胞的旁分泌因子：刺激DNA合成和钠钾ATP酶活性。
Hepatocyte growth factor is a paracrine factor for renal epithelial cells: stimulation of DNA synthesis and NA,K-ATPase activity.
作者信息
Ishibashi K, Sasaki S, Sakamoto H, Nakamura Y, Hata T, Nakamura T, Marumo F
机构信息
Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
出版信息
Biochem Biophys Res Commun. 1992 Jan 31;182(2):960-5. doi: 10.1016/0006-291x(92)91825-b.
The expressions of mRNAs of hepatocyte growth factor (HGF) and its receptor (c-met) and its effects were examined in cultured renal epithelial cell lines (OK, LLCPK1, and MDCK cells) and rat mesangial cells in primary culture. Northern blot analysis revealed the presence of HGF mRNA in mesangial cells, but not in epithelial cells. c-met mRNA was detected in epithelial cells, but not in mesangial cells. HGF stimulated [3H]-thymidine incorporation (DNA synthesis) dose-dependently in OK and LLCPK1 cells, but not in MDCK and mesangial cells. Ouabaine sensitive rubidium uptake (Na,K-ATPase activity) was stimulated by 63% with HGF (10 ng/ml) treatment for 16hr in MDCK cells. The results suggest that HGF is produced in the kidney, at least in mesangial cells and works on epithelial cells to stimulate the proliferation and/or to modify cell functions in a paracrine manner.
在培养的肾上皮细胞系（OK、LLCPK1和MDCK细胞）以及原代培养的大鼠系膜细胞中，检测了肝细胞生长因子（HGF）及其受体（c-met）的mRNA表达及其作用。Northern印迹分析显示系膜细胞中存在HGF mRNA，但上皮细胞中没有。在上皮细胞中检测到c-met mRNA，但系膜细胞中没有。HGF在OK和LLCPK1细胞中剂量依赖性地刺激[3H] - 胸腺嘧啶核苷掺入（DNA合成），但在MDCK和系膜细胞中没有。在MDCK细胞中，用HGF（10 ng/ml）处理16小时后，哇巴因敏感的铷摄取（钠钾ATP酶活性）增加了63%。结果表明，HGF至少在系膜细胞中在肾脏产生，并以旁分泌方式作用于上皮细胞以刺激增殖和/或改变细胞功能。
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