Yamaguchi K, Nalesnik M A, Michalopoulos G K
Division of Transplant Pathology, University of Pittsburgh, School of Medicine, PA 15213, USA.
Urol Res. 1996;24(6):349-54. doi: 10.1007/BF00389792.
In situ hybridization was performed to demonstrate hepatocyte growth factor (HGF) mRNA in two patients with normal kidney and in 23 patients with allograft nephrectomy. In situ hybridization was combined with immunohistochemistry to identify HGF-producing cells. In the two patients with normal kidney, no HGF mRNA was obtainable. In 15 of the 23 allograft patients, signals of HGF mRNA were detectable. In six of these 15 patients, the signals were present mainly at the medullocortex junction, and in the other nine patients at the cortex and/or medulla. Strong and frequent signals were present in gland-like structures in 15 cases. Some scattered signals were also present in the fibrosed glomeruli in five cases, in the thickened intimas of large arteries in three cases, and in the arterial muscle coats of two cases. Combined immunohistochemistry and in situ hybridization showed that HGF mRNA-positive cells in gland-like arrangements were also positive for cytokeratin and negative for factor VIII. Cells with HGF mRNA signal and located in the arterial media were also positive for actin. These findings suggest that HGF mRNA is transcribed both in the urinary tubular epithelium and in the mesenchymal cells (fibroblasts, and smooth muscle cells in chronic vascular rejection and endothelial cells and/or mesangial cells in transplant glomerulopathy) in human rejecting kidney.
对2例肾脏正常的患者及23例接受移植肾切除的患者进行原位杂交，以检测肝细胞生长因子（HGF）mRNA。将原位杂交与免疫组织化学相结合，以识别产生HGF的细胞。在2例肾脏正常的患者中，未检测到HGF mRNA。在23例移植肾患者中的15例，可检测到HGF mRNA信号。在这15例患者中的6例，信号主要出现在髓质皮质交界处，在其他9例患者中出现在皮质和/或髓质。15例中，在腺样结构中出现强烈且频繁的信号。5例中，在纤维化的肾小球中也有一些散在信号；3例中，在大动脉增厚的内膜中有散在信号；2例中，在动脉肌层中有散在信号。免疫组织化学与原位杂交相结合显示，呈腺样排列的HGF mRNA阳性细胞细胞角蛋白也呈阳性，而因子Ⅷ呈阴性。位于动脉中膜且有HGF mRNA信号的细胞肌动蛋白也呈阳性。这些发现提示，在人类移植肾排斥反应中，HGF mRNA在肾小管上皮细胞以及间充质细胞（慢性血管排斥反应中的成纤维细胞和平滑肌细胞，以及移植性肾小球病中的内皮细胞和/或系膜细胞）中均有转录。
