Kramer M D, Simon M M, Tilgen W, Näher H, Justus C W, Petzoldt D
Institut für Immunologie und Serologie, Heidelberg, Germany.
Fertil Steril. 1992 Feb;57(2):417-21.
To determine the broad-spectrum proteinase inhibitor alpha 2-macroglobulin (alpha 2M) and its functional subforms, i.e., free alpha 2M and alpha 2M/proteinase complexes, in human seminal fluid by using specific enzyme immunoassay systems.
The study has been performed in the Andrology Department of the Dermatology Clinics and the Laboratory of Immunopathology of the Institute of Immunology. PATIENTS, PARTICIPATES: Routine patients attending the Andrology Department.
The data have been obtained without particular interventions before or after collection of seminal fluid.
The aim of the study was to determine whether alpha 2M or alpha 2M/proteinase complexes are present in human seminal fluid.
The concentration of total alpha 2M in human seminal fluid ranged from 1 to greater than 1,000 ng/mL, and between 56% and 85% of the inhibitor was complexed with proteinases.
These findings show that alpha 2M and alpha 2M/proteinase complexes have to be considered as functionally relevant biomolecules in male genital tract secretions.
通过使用特定的酶免疫分析系统，测定人精液中的广谱蛋白酶抑制剂α2-巨球蛋白（α2M）及其功能亚形式，即游离α2M和α2M/蛋白酶复合物。
该研究在皮肤病诊所男科和免疫学研究所免疫病理学实验室进行。
患者、参与者：男科门诊的常规患者。
在采集精液之前或之后，无需特殊干预即可获得数据。
该研究的目的是确定人精液中是否存在α2M或α2M/蛋白酶复合物。
人精液中总α2M的浓度范围为1至大于1000 ng/mL，并且56%至85%的抑制剂与蛋白酶结合。
这些发现表明，α2M和α2M/蛋白酶复合物必须被视为男性生殖道分泌物中功能相关的生物分子。
