Feng X H, Bottino P J, Kung S D
Center for Agricultural Biotechnology, University of Maryland, College Park 20742-5815.
Plant Mol Biol. 1992 Feb;18(3):581-4. doi: 10.1007/BF00040673.
In this study we report identification of six members of a protein kinase gene family from soybean (Glycine max L.). Two fully degenerate oligonucleotide primers corresponding to two conserved motifs (DLK-PENV and GTHEYLAPE) in the catalytic domains of eukaryotic protein serine/threonine kinases were used in a polymerase chain reaction (PCR) to amplify soybean cDNA. Sequence analysis showed that 28 of the PCR sequences represented six different putative protein serine/threonine kinases. These results not only demonstrate that catalytic domains of protein kinases are highly conserved between plants and other eukaryotes but also suggest that there are multiple genes encoding protein kinases in plants.
在本研究中，我们报告了从大豆（Glycine max L.）中鉴定出一个蛋白激酶基因家族的六个成员。对应于真核蛋白丝氨酸/苏氨酸激酶催化结构域中两个保守基序（DLK-PENV和GTHEYLAPE）的两个完全简并的寡核苷酸引物用于聚合酶链反应（PCR）以扩增大豆cDNA。序列分析表明，28个PCR序列代表六种不同的假定蛋白丝氨酸/苏氨酸激酶。这些结果不仅证明了植物和其他真核生物之间蛋白激酶的催化结构域高度保守，而且还表明植物中存在多个编码蛋白激酶的基因。
