Silver P A
Department of Molecular Biology, Princeton University, New Jersey 08544.
Cell. 1991 Feb 8;64(3):489-97. doi: 10.1016/0092-8674(91)90233-o.
Nuclear protein import is a selective process. Proteins destined for the nucleus contain NLSs. These short stretches of amino acids interact with proteins located in the cytoplasm, on the nuclear envelope, and/or at the nuclear pore complex. Following binding at the pore complex, proteins are translocated through the pore into the nucleus in a manner requiring ATP. The biochemical dissection of the nuclear pore complex has begun. Alteration of protein import into the nucleus is emerging as a new and complex form of regulation. However, we are left with the following problems: How do proteins move through the cytoplasm to reach the nuclear pore? How does the nuclear pore complex open and close in a selective manner? How is ATP utilized during import? And finally, how is bi-directional traffic of both proteins and RNA through the pore regulated?
核蛋白输入是一个选择性过程。 destined for the nucleus contain NLSs. These short stretches of amino acids interact with proteins located in the cytoplasm, on the nuclear envelope, and/or at the nuclear pore complex. Following binding at the pore complex, proteins are translocated through the pore into the nucleus in a manner requiring ATP. The biochemical dissection of the nuclear pore complex has begun. Alteration of protein import into the nucleus is emerging as a new and complex form of regulation. However, we are left with the following problems: How do proteins move through the cytoplasm to reach the nuclear pore? How does the nuclear pore complex open and close in a selective manner? How is ATP utilized during import? And finally, how is bi-directional traffic of both proteins and RNA through the pore regulated?
核蛋白的输入是一个选择性过程。进入细胞核的蛋白质含有核定位信号（NLSs）。这些短的氨基酸序列与位于细胞质、核膜和/或核孔复合体上的蛋白质相互作用。在与孔复合体结合后，蛋白质以需要ATP的方式通过孔转运到细胞核中。对核孔复合体的生化剖析已经开始。核蛋白输入的改变正在成为一种新的、复杂的调节形式。然而，我们面临以下问题：蛋白质如何穿过细胞质到达核孔？核孔复合体如何以选择性方式打开和关闭？在输入过程中ATP是如何被利用的？最后，蛋白质和RNA通过孔的双向运输是如何被调节的？
