Distamycin A及一些小沟结合剂对博来霉素催化的位点特异性DNA切割的增强和改变作用。

Enhancement and alteration of bleomycin-catalyzed site-specific DNA cleavage by distamycin A and some minor groove binders.

作者信息

Yamamoto K, Kawanishi S

机构信息

Department of Public Health, Faculty of Medicine, Kyoto University, Japan.

出版信息

Biochem Biophys Res Commun. 1992 Feb 28;183(1):292-9. doi: 10.1016/0006-291x(92)91642-4.

DOI:10.1016/0006-291x(92)91642-4

PMID:1371920

Abstract

The effects of compounds which bind in the DNA minor groove of A.T rich sequences, on bleomycin-catalyzed site-specific DNA cleavage were investigated by a DNA sequencing technique. Distamycin A enhanced bleomycin-catalyzed DNA cleavage in G.C rich sequences such as 5'-GGGGC-3' (under scoring; the cleaved nucleotide). The cleavage in such a sequence in the presence of distamycin A was greater than that in the absence of distamycin A by as much as about 100 times. Neither Hoechst 33258, 4',6-diamidino-2-phenylindole (DAPI) nor berenil caused extensive enhancement. The results suggest that the distamycin-induced conformational changes of DNA through interactions other than the DNA minor groove binding in A.T-rich sequences are specifically suitable for the bleomycin action.

摘要

通过DNA测序技术研究了与富含A.T序列的DNA小沟结合的化合物对博来霉素催化的位点特异性DNA切割的影响。Distamycin A增强了博来霉素对富含G.C序列（如5'-GGGGC-3'，下划线表示切割的核苷酸）的DNA切割作用。在存在Distamycin A的情况下，该序列中的切割比不存在Distamycin A时增强了约100倍。Hoechst 33258、4',6-二脒基-2-苯基吲哚（DAPI）和贝尼尔均未引起广泛增强。结果表明，Distamycin通过与富含A.T序列中DNA小沟结合以外的相互作用诱导的DNA构象变化特别适合博来霉素的作用。