Sengar D P, Rashid A, Harris J E
Int Arch Allergy Appl Immunol. 1976;51(6):732-41. doi: 10.1159/000231651.
The in vitro response of lymphocytes obtained from normal subjects, uraemic patients on haemodialysis and diabetic patients was studied using cultures containing either medium plus plasma (medium cultures) or plasma alone (plasma cultures). The study demonstrated that plasma alone can adequately support lymphocyte transformation induced by nonspecific mitogens (PHA and PWM) and allogeneic lymphocytes in mixed lymphocyte culture (MLC) reaction. This investigation further confirms our previously reported findings that uraemic patients undergoing haemodialysis have a normal lymphocyte response in MLC and to PHA and PWM. Plasma cultures give results similar to conventional medium cultures in subjects where lymphocyte transformation is normal. The lymphocyte hyporactivity observed in diabetics is, however, better shown in the plasma cultures. The suppressed response of the diabetic patient's lymphocytes to PHA and PWM both in the presence of autologous and normal AB plasma suggests intrinsic lymphocyte dysfunction as the explanation for impaired immune function. Plasma cultures may provide a better in vitro system for the evaluation of immune function in certain groups of patients where it is desirable to distinguish between intrinsic abnormalities of lymphocyte function and the effect of humoral immunosuppressive factors.
使用含有培养基加血浆（培养基培养）或仅血浆（血浆培养）的培养物，研究了从正常受试者、接受血液透析的尿毒症患者和糖尿病患者获得的淋巴细胞的体外反应。该研究表明，仅血浆就可以充分支持非特异性有丝分裂原（PHA和PWM）诱导的淋巴细胞转化以及混合淋巴细胞培养（MLC）反应中同种异体淋巴细胞的转化。这项研究进一步证实了我们先前报道的结果，即接受血液透析的尿毒症患者在MLC中以及对PHA和PWM具有正常的淋巴细胞反应。在淋巴细胞转化正常的受试者中，血浆培养的结果与传统培养基培养的结果相似。然而，糖尿病患者中观察到的淋巴细胞低活性在血浆培养中表现得更明显。糖尿病患者淋巴细胞在自体和正常AB血浆存在下对PHA和PWM的反应受到抑制，这表明淋巴细胞内在功能障碍是免疫功能受损的原因。血浆培养可能为评估某些患者群体的免疫功能提供一个更好的体外系统，在这些患者中，区分淋巴细胞功能的内在异常和体液免疫抑制因子的作用是很有必要的。
