Ditscherlein G, Schneider W
Int Urol Nephrol. 1976;8(3):227-35. doi: 10.1007/BF02082244.
One hundred renal biopsy specimens out of the routine material were investigated by the usual paraffin technique, by semi-thin sections and by the immunofluorescence technique. The findings were compared in regard of the diagnoses made by each technique. The results indicate that the paraffin technique is inadequate in a considerable proportion of the specimens, because very small glomerular lesions cannot be recognized. The semi-thin section technique enabled us to correct the light-microscopic diagnosis in 17 per cent, particularly in establishing membranous glomerulonephritis. In another 31 per cent the histologic diagnoses could be improved. The immunofluorescence technique provided evidence of active glomerulonephritis in 57 per cent (24 cases with immune complex nephritis and 3 cases with basement membrane antibody nephritis). We found a much closer correlation between results of the semi-thin section technique and immunohistology that between the paraffin technique and immunohistology.
采用常规石蜡切片技术、半薄切片技术和免疫荧光技术，对100份常规材料中的肾活检标本进行了研究。对每种技术所做的诊断结果进行了比较。结果表明，在相当一部分标本中，石蜡切片技术并不充分，因为非常小的肾小球病变无法识别。半薄切片技术使我们能够在17%的病例中修正光镜诊断，特别是在确诊膜性肾小球肾炎方面。在另外31%的病例中，组织学诊断可以得到改善。免疫荧光技术在57%的病例中（24例免疫复合物性肾炎和3例基底膜抗体性肾炎）证实存在活动性肾小球肾炎。我们发现，半薄切片技术与免疫组织学结果之间的相关性比石蜡切片技术与免疫组织学结果之间的相关性更为密切。
