Bobolis K A, Moulds J J, Telen M J
Department of Medicine, Duke University Medical Center, Durham, NC 27710.
Blood. 1992 Mar 15;79(6):1574-81.
JMH is a high-frequency human erythrocyte blood group antigen. Previous work has shown that JMH is absent from complement-sensitive erythrocytes of patients with paroxysmal nocturnal hemoglobinuria (PNH); such cells have a broad defect in expression of phosphatidylinositol (PI)-linked proteins. Using both human JMH antisera and a JMH-like murine monoclonal antibody, we have identified a 76-Kd membrane protein present in JMH-positive but not JMH-negative erythrocytes. A similar 76-Kd JMH protein was also identified on a human lymphoid T-cell line, HSB-2. Using PI-specific phospholipase C, a small amount of JMH antigen could be cleaved from intact erythrocytes and immunoprecipitated from the supernate of treated erythrocytes, thus confirming that the protein bearing the JMH antigen is anchored by a PI-linkage to the erythrocyte membrane. This protein was further shown not to be identical to decay accelerating factor (70 Kd), a previously identified PI-anchored protein of somewhat similar molecular weight.
JMH是一种高频人类红细胞血型抗原。先前的研究表明，阵发性夜间血红蛋白尿（PNH）患者对补体敏感的红细胞中不存在JMH；这类细胞在磷脂酰肌醇（PI）连接蛋白的表达上存在广泛缺陷。利用人JMH抗血清和一种类似JMH的鼠单克隆抗体，我们在JMH阳性而非JMH阴性的红细胞中鉴定出一种76千道尔顿的膜蛋白。在人淋巴T细胞系HSB-2上也鉴定出了类似的76千道尔顿JMH蛋白。使用PI特异性磷脂酶C，可从完整红细胞上裂解下少量JMH抗原，并从处理过的红细胞上清液中免疫沉淀出来，从而证实携带JMH抗原的蛋白通过PI连接锚定在红细胞膜上。进一步研究表明，该蛋白与衰变加速因子（70千道尔顿）不同，衰变加速因子是先前鉴定出的一种分子量稍相似的PI锚定蛋白。
