Athanassakis-Vassiliadis I, Vassiliadis S, Papamatheakis J
Institute of Molecular Biology and Biotechnology, Heraklion, Crete, Greece.
J Reprod Immunol. 1992 Mar;21(2):149-61. doi: 10.1016/0165-0378(92)90021-u.
It has been shown that there is an inverse as well as a direct correlation between class II MHC antigen expression and the p21ras protein, depending on the cell type. By using trophoblastic cells derived from the spongiotrophoblast and labyrinthine trophoblast, the two major components of the murine placenta, we have examined the p21ras expression in these populations and how this correlates with the induction of class II MHC antigens. It has been shown that although only a small number of cells express the p21ras and class II proteins, they can be induced to express higher levels of these markers by two different treatments. Thus, gamma-interferon (gamma-IFN) induces p21ras and class II protein expression in spongiotrophoblast-derived cells, whereas 5-Azacytidine (5-AzaC) induces expression of these antigens in labyrinthine trophoblast-derived populations. Furthermore, it has been demonstrated that there is a direct correlation between the two markers, as blocking antibody to p21ras cancels the ability of gamma-IFN and 5-AzaC to induce class II antigens. As previous work from this laboratory has shown that in vivo class II induction in the placenta leads to fetal abortion, the present results suggest that both proteins are involved in a common signal transduction pathway which may lead to disruption of fetal membranes and fetal loss.
已经表明，根据细胞类型，II类MHC抗原表达与p21ras蛋白之间存在负相关以及正相关。通过使用源自海绵滋养层和迷路滋养层（小鼠胎盘的两个主要组成部分）的滋养层细胞，我们研究了这些细胞群体中p21ras的表达情况以及它与II类MHC抗原诱导之间的相关性。已经表明，虽然只有少数细胞表达p21ras和II类蛋白，但通过两种不同的处理可以诱导它们表达更高水平的这些标志物。因此，γ-干扰素（γ-IFN）可诱导海绵滋养层来源的细胞中p21ras和II类蛋白的表达，而5-氮杂胞苷（5-AzaC）可诱导迷路滋养层来源的细胞群体中这些抗原的表达。此外，已经证明这两种标志物之间存在正相关，因为针对p21ras的阻断抗体可消除γ-IFN和5-AzaC诱导II类抗原的能力。由于该实验室先前的研究表明，胎盘中的II类诱导在体内会导致胎儿流产，目前的结果表明这两种蛋白都参与了一个共同的信号转导途径，该途径可能导致胎膜破裂和胎儿丢失。
