从小鼠红白血病细胞中克隆D型细胞周期蛋白。
Cloning of a D-type cyclin from murine erythroleukemia cells.
作者信息
Kiyokawa H, Busquets X, Powell C T, Ngo L, Rifkind R A, Marks P A
机构信息
DeWitt Wallace Research Laboratory, Memorial Sloan-Kettering Cancer Center, New York, NY.
出版信息
Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2444-7. doi: 10.1073/pnas.89.6.2444.
We report the complete coding sequence of a cDNA, designated CYL2, derived from a murine erythroleukemia cell library. CYL2 is considered to encode a D-type cyclin because (i) there is cross hybridization with CYL1 (a murine homolog of human cyclin D1) and the encoded protein has 64% amino acid sequence identity with CYL1 and (ii) murine erythroleukemia cell-derived CYL2 contains an amino acid sequence identical to that previously reported for the C-terminal portion of a partially sequenced CYL2. Transcripts of murine erythroleukemia cell CYL2 undergo alternative polyadenylylation like that of human cyclin D1. A major 6.5-kilobase CYL2 transcript changes its expression during the cell cycle with a broad peak through G1 and S phases and a decrease in G2/M phases. The present findings suggest that CYL2 plays a role in the G1 to S phase progression.
我们报道了一个来自小鼠红白血病细胞文库的cDNA（命名为CYL2）的完整编码序列。CYL2被认为编码一种D型细胞周期蛋白，原因如下：（i）它与CYL1（人细胞周期蛋白D1的小鼠同源物）存在交叉杂交，且编码的蛋白质与CYL1具有64%的氨基酸序列同一性；（ii）小鼠红白血病细胞来源的CYL2包含的氨基酸序列与先前报道的部分测序的CYL2的C末端部分相同。小鼠红白血病细胞CYL2的转录本像人细胞周期蛋白D1的转录本一样经历可变聚腺苷酸化。一个主要的6.5千碱基的CYL2转录本在细胞周期中改变其表达，在G1期和S期有一个宽泛的峰值，在G2/M期减少。目前的研究结果表明CYL2在G1期到S期的进程中发挥作用。
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