利用互补作用克隆了裂殖酵母细胞周期控制基因cdc2的一个人类同源基因。

Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.

作者信息

Lee M G, Nurse P

出版信息

Nature. 1987;327(6117):31-5. doi: 10.1038/327031a0.

Abstract

A human homologue of the cdc2 gene has been cloned by expressing a human cDNA library in fission yeast and selecting for clones that can complement a mutant of cdc2. The predicted protein sequence of the human homologue is very similar to that of the yeast cdc2 gene. These data indicate that elements of the mechanism by which the cell cycle is controlled are likely to be conserved between yeast and humans.

摘要

通过在裂殖酵母中表达人cDNA文库并筛选能够互补cdc2突变体的克隆，已克隆出cdc2基因的人同源物。该人同源物的预测蛋白质序列与酵母cdc2基因的序列非常相似。这些数据表明，细胞周期调控机制的元件在酵母和人类之间可能是保守的。