Armstrong E, Vainikka S, Partanen J, Korhonen J, Alitalo R
Department of Virology, University of Helsinki, Finland.
Cancer Res. 1992 Apr 1;52(7):2004-7.
We have previously cloned from K562 leukemia cells two novel fibroblast growth factor receptors (FGFR-3 and FGFR-4; J. Partanen et al., EMBO J., 10: 1347-1354, 1991). Here we have analyzed the mRNA expression of four different FGFRs, including the two novel genes in human leukemia cell lines. We show FGFR-1, FGFR-3, and FGFR-4 mRNAs in several leukemia cell lines at levels similar to those in solid tumor cell lines. Ligand cross-linking experiments indicate that K562 cells have receptors binding acidic FGF but not basic FGF. Expression of FGFRs in leukemia cells may reflect their presence on normal hematopoietic precursor cells or induction during leukemogenesis or cell culture.
我们先前已从K562白血病细胞中克隆出两种新型成纤维细胞生长因子受体（FGFR-3和FGFR-4；J. 帕尔塔宁等人，《欧洲分子生物学组织杂志》，10: 1347 - 1354, 1991）。在此，我们分析了四种不同FGFRs的mRNA表达情况，包括这两个新基因在人白血病细胞系中的表达。我们发现几种白血病细胞系中FGFR-1、FGFR-3和FGFR-4的mRNA水平与实体瘤细胞系中的水平相似。配体交联实验表明，K562细胞具有结合酸性FGF的受体，但不结合碱性FGF。白血病细胞中FGFRs的表达可能反映了它们在正常造血前体细胞上的存在，或者在白血病发生过程或细胞培养过程中的诱导情况。
