Waters S H, O'Neil J J, Melican D T, Appel M C
Department of Pathology, University of Massachusetts Medical School, Worcester.
Diabetes. 1992 Mar;41(3):308-12. doi: 10.2337/diab.41.3.308.
Because a restricted repertoire of T-cell receptor (TCR) V beta gene expression has been reported in other autoimmune diseases, the possibility of similarly restricted V beta gene expression by T-cell infiltrates of NOD mouse islets was examined. With isolated islets from 4- to 12-wk-old NOD mice, a prospective polymerase chain reaction analysis with 18 V beta-specific oligonucleotide primers was performed on the noncloned and unexpanded islet-infiltrating T cells. The methodology used permitted the detection of a minimum of 50 T cells. In contrast to the restricted TCR V beta gene usage reported for other autoimmune diseases, infiltrates of even the youngest mice were characterized by expression of multiple V beta gene segments.
由于在其他自身免疫性疾病中已报道了T细胞受体（TCR）Vβ基因表达谱受限，因此研究了NOD小鼠胰岛T细胞浸润中Vβ基因表达同样受限的可能性。利用4至12周龄NOD小鼠的分离胰岛，对未克隆和未扩增的胰岛浸润T细胞进行了18种Vβ特异性寡核苷酸引物的前瞻性聚合酶链反应分析。所使用的方法能够检测到至少50个T细胞。与其他自身免疫性疾病报道的受限TCR Vβ基因使用情况相反，即使是最年幼小鼠的浸润细胞也以多个Vβ基因片段的表达为特征。
